Abstract

AUGMENTATION OF TNF-?, COX-2, CASPASE-3, CD95 EXPRESSION IN MICE LIVER CAUSED BY AZORUBINE ADMINISTRATION AND THE IMMUNOMODULATORY EFFECT OF VITAMIN E

Amal A. E. Ibrahim*, Sally A. El- Sherbiny, Engy A. El-Sherbiny and Amira H. El-Etriby

ABSTRACT

The present study aimed to evaluate the mechanism of action for vitamin E against hepatotoxicity by food additive azorubine in male young mice. Mice divide into 4 groups: group I was the control; group II received vitamin E (100mg/kg); group III received azorubine (250mg/kg) and group IV received vitamin E+azorubine for 28 days. Hepatotoxicity occurred as a result of increased hepatic markers, changes in antioxidant activities, increased of TNF-α, COX, caspase-3, CD95 and decreased of Bcl-2 concentrations. Hepatic tissue showed degeneration and pyknosis due to the administration of azorubine. Injection of vitamin E caused significant restoration in the levels of liver functions, antioxidants status, histological architecture in liver \ and improvement in the immunological parameters (TNF-α, Cox-2, caspase-3, CD95 and Bcl-2). The preventive role of vitamin E against azorubine induced toxicity attributed to its powerful antioxidant role, free radical scavenging properties and its immunomodulatory effect.

Keywords: Hepatotoxicity – Azorubine - Vitamin E – Mice.