Abstract

MICELLAR ELECTROKINETIC CHROMATOGRAPHY – A REVIEW

Sugandhi Puvvadi, Nikitha Chakala, S. Varalaxmi* and J. Neha Venkata Durga

ABSTRACT

Capillary electrophoresis (CE) is an alternative analytical technique for the pharmaceutical analysis in parallel with liquid chromatography. Micellar electrokinetic chromatography is based on the addition of a micellar pseudo stationary phase to the buffer solution, which interacts with the analytes according to partitioning mechanism, like in chromatography method. Basic principles involved are electrophoretic mobility and electroosmotic flow. In Micellar electrokinetic chromatography common sample introduction methods are hydrodynamic injection method and electrokinetic injection method. Micellar electrokinetic chromatography is a hybrid of electrophroesis and chromatography This mechanism is based on separation of ions by using applied voltage. In this, using some of detectors like UV,PDA,CIF, Laser induced fluorescence, electrochemical and mass spectrometric (MS) detection. Characteristics of MEKC are nanotechnique, high–speed, high-resolution, high sensitivity, no molecular weight limitation, mild conditions and compatibility with biological systems.

Keywords: Capillary electrophoresis, Micellar electrokinetic chromatography, Pseudo stationary phase.