Abstract

FORMULATION AND EVALUATION OF ANTIMICROBIAL HERBAL CREAM USING AQUEOUS EXTRACT OF GUAVA LEAF

A. C. Menkudale*, T. N. Pasalkar, P. P. Shinde, P. S. Ubale, R. V. Punde and M. S. Jadhav

ABSTRACT

The topical cream is an effective & painless way to kill the micro-organisms and various bacteria present on the skin. In the present study herbal antimicrobial cream was prepared by using aqueous guava leaf extract. Pharmacognostical study of guava leaf was performed. Further aqueous extract of guava leaves was prepared by maceration technique. Phytochemical analysis of aqueous extract of guava leaves was performed for determining presence of various Phytoconstituents. Presence of tannins helps to indicates chances of antimicrobial property of guava leaf. The Agar diffusion method was used to determine anti-microbial activity of aqueous guava leaf extract. Two bacterial cultures namely E-coli (gram negative bacteria) and staphylococcus aurous (gram positive bacteria) were used for determination of antimicrobial property of extract. Herbal cream was prepared by using guava leaf extract. Prepared cream was evaluated for various parameters such as colour, odour, pH, spreading coefficient, skin irritancy, dye test, phase separation, saponification value, moisture absorption, Removal test & stability etc.

Keywords: Herbal cream, Extract, Phytochemical, Anti microbial, Bacteria, Phase separation, etc.