Abstract

QUANTIFICATION OF GALLIC ACID BY A VALIDATED HPTLC METHOD IN A POLYHERBAL FORMULATION: KARKATI BEEJ CHURNA

Dalal Ketaki Pravin* and Tekale Prafullachandra

ABSTRACT

Quantification and validation of active components using analytical concepts in an herbal formulation is an important parameter to qualify and authenticate herbal medications. As per the reference text; Brihad Nighantu Ratnakara, Karkatibeej churna is a polyherbal formulation which is used to treat urinary disorders and contains components which are diuretic in nature. In the present study, an attempt was made to qualify, quantify and validate Gallic acid, a phenolic compound which is diuretic and anti-inflammatory in nature by High performance thin layer chromatography (HPTLC). Methanolic extracts of churna formulation were applied on the plate and the plate was developed in the mobile phase toluene: ethyl acetate: formic acid (5:4:1 v/v/v). The Rf value of standard and sample matched at 0.31. The HPTLC method was validated for linearity, accuracy (recovery), precision, Limit of detection (LOD) and Limit of quantification (LOQ), robustness and specificity. The linearity for the calibration range of 150ng-350ng gave good linear relationship with r= 0.999615 and coefficient of variation of 0.85%. LOD and LOQ were estimated to be 22.95 ng and 48.77 ng respectively. Gallic acid recovery and %CV for intraday precision was obtained to be 101.19% and 2.16% respectively. The amount of Gallic acid estimated in Karkatibeej churna formulation was 102.8 μg /20 mg. The developed method was robust and specific, simple, accurate, precise and suitable for fingerprinting and quantification of Gallic acid in Karkatibeej churna formulation.

Keywords: Gallic acid, HPTLC, Karkatibeej Churna, Diuretic, Antiinflammatory, Polyherbal.