Abstract

IN SILICO ANALYSIS OF GENES AND PATHWAYS RELATED TO ACUTE MYELOID LEUKEMIA PROMOTING LEUKOPENIA

Ajeet Kumar, Ravi Bhushan, Pawan K. Dubey, Vijai Tilak, Vineeta Gupta, Nilesh Kumar, S.V.S Raju and Akhtar Ali*

ABSTRACT

Acute myeloid leukemia (AML) is a type of blood cancers that begins from progenitor and hematopoietic stem cell. Chromosomal abnormalities include balanced translocations between two chromosome like t[8;21] and t[15;17]) in malignancies cells. The present study aimed to explore the AML presenting with leukopenia and gene expression changes induced in High-white count B-cell and Low-white count B-cell the total number of samples is ten. The raw gene expression profiles (ID: GSE20482) of bone marrow achieve from AML patient five High-white count B-cell and five Low-white count B-cell were expressed genes used to recognize differentially. These genes that correspond to official gene symbols were select for protein-protein interaction (PPI) and sub-network construction (score > 0.4). The functional annotation of Gene Ontology (GO) and pathways analysis were performed for those genes involve in networking. Results: The total number of 846 genes were identified differentially expressed gene and 406 gene were up-regulated another 440 gene were down-regulated. Other 14 genes are interacting with each other significantly identified. Including Hub genes DEGs GNB4, LAMTOR2, ACTN4, HGSNAT, TMED1 are up-regulated while down-regulated DEGs forming hub nodes were UBR4, FBXO30, KLHL21, DCTN6, RNF123, RNF114. AML has a major effect on the expression of genes involved in cell differentiation, apoptosis, cell signaling and modification of protein. AML cells enter the blood quickly and spread to the liver, spleen, and central nervous system. These are total thirteen pathways were enriched and these genes related to oxidative phosphorylation, regulation of actin cytoskeleton, endocytosis, phagocytosis, shigellosis, epithelial cell signaling in helicobacter, adherent junction, pertussis, bile secretion, malaria, African trypanosomiasis were found significantly affected by AML. Conclusions: Hub genes like GNB4 and UBR4 provide as a novel biomarker in AML.

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