Abstract

A REVIEW ON HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD DEVELOPMENT AND VALIDATION

Prashant Kashinath Wankhede*, Pooja Gopalsing Bedwal, Dipak Buchake, Dr. Lahu D. Hingane, Dr. Prachi P. Udapurkar

ABSTRACT

This abstract summarizes the development and validation of an analytical method using High-Performance Thin-Layer Chromatography (HPTLC). The process involves designing and optimizing the HPTLC method for the separation, identification, and quantification of target compounds in a sample. Method validation ensures that the developed HPTLC method is reliable, accurate, and precise. Various validation parameters, such as linearity, specificity, accuracy, precision, robustness, and system suitability, are evaluated to establish the method's performance characteristics. The development and validation of HPTLC methods play a crucial role in ensuring the quality and reliability of analytical results, making them an essential step in analytical research and routine analysis.

Keywords: Analytical method, development, validation, High-Performance Thin-Layer Chromatography (HPTLC), separation, identification, quantification, optimization, linearity, specificity, accuracy, precision, robustness, system suitability, quality, reliability, re