Abstract

SYNTHESIS AND BIOLOGICAL EVALUATION OF NEW DERIVATIVES OF PYRAZOLINE

Bebi S.*, Desai S., Prasad Bali and Sharanagoud Biradar

ABSTRACT

New pyrazoline derivatives have been synthesized in two steps. Using 2-acetyl indane 1,3 dione and various aromatic aldehydes as reactants, the Claisen-Schmidt reaction was used to synthesize chalcone in the initial step of the process. The chalcone was converted to pyrazolines in the second step by cyclization in acidic medium with a few hydrazine derivatives. By determining the physical, chromatographic, spectroscopic and elemental analysis, all the compounds were confirmed. Chalcones and their 2-Amino-Pyrazoline derivatives of indane-1,3-dione (1a-d and 2a-d) were tested for their anti-bacterial against a gram-positive bacterium viz. Staphylococcus mutans and a gram-negative bacterium viz. Eshcherichia coli by following disk-diffusion method. Ciprofloxacin was used as a standard drug for antibacterial activity. All the compounds have shown moderate to good antibacterial activity against E. coli. (MIC 25μg/ml). Compounds 1c and 2c have exhibited more anti-bacterial activity. Antifungal activity of compounds 1c and 2c against candida albicans (MIC 25μg/ml) was found to be better compared to all other compounds; fluconazole was used as a standard drug.

Keywords: 2- Acetyl-indane- 13- dione, Chalcones, Pyrozoline, Disc Diffusion Method.