Abstract

PHYTOCHEMICAL AND ANTIDIABETIC OF PROSOPIS JULIFLORA LEAVES ON ALLOXON INDUCED MODEL

K. Hemamalini* and Kanathala Madhulika

ABSTRACT

This research aims to investigate the antidiabetic activity of Prosopis juliflora leaf extract in alloxan induced diabetic albino rats. A comparison was made between the action of Prosopis juliflora extracts and a known antidiabetic drug glibenclamide (600 μg/kg body wt.). An oral glucose tolerance test (OGTT) was also performed in experimental diabetic rats. The petroleum ether, chloroform, alcohol and aqueous extracts of Prosopis juliflora were obtained by simple maceration method and subjected to standardization by following pharmacognostical and phytochemical screening methods. Dose selection was made on the basis of acute oral toxicity study (50mg to 5000 mg/kg body weight) as per OECD guidelines. Prosopis juliflora chloroform extract (PJCE) and ethanolic extract (PJEE) showed significant (P<0.001) antidiabetic activity. In alloxan induced model, blood glucose level of these extracts on the seventh day of study were PJCE (113.33 ± 6.662) and PJEE (169.33 ± 9.735) in comparison of diabetic control (411.50 ± 3.752) and petroleum ether extract (328.83 ± 23.515). In OGGT model (glucose loaded rats), PJCE exhibited glucose level after 30 min. (162.33 ± 5.661) and 90 min. (119.00 ± 2.966) whereas PJEE after 30 min. (173.16 ± 3.380) and 90 min. (128.00 ± 5.266). These extracts also prevented body weight loss in diabetic rats. The antihyperglycemic action of the extracts may be due to improving the glycemic control mechanisms. The drug has the potential to act as antidiabetic drug.

Keywords: Acute toxicity, Alloxan monohydrate, Antidiabetic activity, Prosopis juliflora.