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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.485

ICV : 84.65

WJPPS Citation

	All	Since 2020
Citation	6651	4087
h-index	26	21
i10-index	174	83

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WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

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Abstract

REVIEW ARTICLE ON HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) METHOD DEVELOPMENT AND VALIDATION

Prince Kumar*

ABSTRACT

Chromatographers face the need to develop a high-performance liquid chromatography (HPLC) method everyday. In this review systematic procedures for developing an HPLC method, based on the best information available. HPLC method development involves sample pre-treatment, choosing separation conditions, detection of sample bands, quantitation, and method validation. HPLC is an analytical tool which is able to detect, separate and quantify the drug, its various impurities and drug related degrades that can form on synthesis or storage. Validation of HPLC method gives information regarding various stages like Accuracy, specificity, linearity limit of detection, limit of quantification.

Keywords: HPLC, mobile phase, stationary phase, method development, validation.

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