Abstract

CONDITIONS INVOLVING THE TOXICITY OF TETRODOTOXIN FROM CARCINOSCORPIUS ROTUNDICAUDA

Canh Le V., Phuc Hua N., Phong Le V. and Tu Nguyen HK.*

ABSTRACT

A famous neurotoxin as tetrodotoxin (TTX) which was found in Carcinoscorpius rotundicauda. However, we do not know clearly when and which environment TTX will show more toxicity and we always wonder about the TTX producing sources. Therefore, the egg fluid of Carcinoscorpius rotundicauda samples was withdrawn and added in different kinds of buffer which were KH2PO4 (pH 7.2), NaCl (1.0, 2.0, 3.0, 4.0%) and distilled water. All of media were let at 25 oC and 37oC in 24 and 72 hours. The extracts were collected by centrifugation to remove the pellet and divided into two parts. One part was heated at 105oC before injected into mice by intraperitoneal route. The injected mice were observed the symptoms and recorded the death duration. The results notified that there was not significant difference when culturing egg fluid in the media in 24 hours but obvious in 72 hours. NaCl (1.0 %) gave the highest effect when comparing to other concentrations. The finding contributes to the TTX information for clarifying the TTX producing sources and TTX relating research for science.

Keywords: Carcinoscorpius rotundicauda, tetrodotoxin, mice bioassay, elisa assay, buffer.