Abstract

INVESTIGATION OF IN-VITRO THERAPEUTIC ACTIVITIES OF METHANOLIC EXTRACT OF JEQUIRTY SEEDS

S. Dharmaraj Santhosam*, P. Selvam and Abhinandan Danodia

ABSTRACT

Abrus precatorius is commonly called Jequrity. This research was designed to investigate the Invitro Antioxidant activity, Antibacterial, Antifungal, Antidiabetic, Anti-inflammatory, and cytotoxicity activity against Vero cells, Hep G2 hepatic cancer cells, and SK-N-SH-Neuroblastoma cells. The crude extract of the powdered jequirity seeds was obtained by the cold maceration method for 7 days by using methanol as the solvent. The crude extract was named CMME. The compounds from the crude extract were separated by column chromatography using silica gel 60-120 mesh as the adsorbent. The isolated compounds are purified by thin-layer chromatography. The fractions collected from the bottom of the column are mixed based on the Rf value. The fractions that have the same Rf value are mixed and the solvents are evaporated and dried under a vacuum. The crude extract was named CMME, The isolated compounds were named CMME I, CMME II, and CMME III according to the first, second, and third eluted compounds from the column. The IC50 value for the antioxidant activity of CMME was calculated for the DPPH Assay method, IC50 value for the anticancer activity of CMME and the isolated compounds CMME I, CMME II, and CMME III was calculated for the MTT assay method against Hep G2 hepatic cancer cell line, The IC50 value for cytotoxicity activity for normal Vero cells was calculated for the CMME and its first isolated compound CMME I. The IC50 value for the anticancer activity for SK-N-SH neuroblastoma cell lines was calculated for the crude extract CMME. The In-vitro antidiabetic, anti-inflammatory activity, antibacterial, and antifungal activity were determined for the CMME and its isolated compounds CMME I, CMME II, and CMME III. The In-vitro antidiabetic activity was determined by the alpha-amylase enzyme inhibition method. The In-vitro anti-inflammatory activity was determined by the protein denaturation method. The antifungal and antibacterial activity was determined by the disc diffusion method.

Keywords: Cytotoxicity, Antioxidant, Anti-inflammatory, Anti-diabetic, Anti-bacterial, and Anti-fungal.