Abstract

FORMULATION AND EVALUATION OF PHYLLANTHIN WITH PIPERINE NANOSUSPENSION FOR ENHANCING THE SOLUBILITY AND DISSOLUTION RATE

Praveen Kumar C., Padmapreetha J. and Arivukkarasu R.*

ABSTRACT

Nanosuspension drug delivery system technique to improve solubility, dissolution rate of herbal drugs. Phyllanthin a poorly water soluble herbal drug obtained from Phyllanthus amarus (Euphorbiaceae). Phyllanthin along with piperine as natural bioenhancer was formulated as nanosuspension using high speed homogenization technique. Three different formulations were prepared using phyllanthin: piperine in different ratio (1:0, 1:0.5, 1:1) with 2% w/v PVA solution as stabilizer. The prepared nanosuspension of three different formulations were lyophilized using 2% mannitol as cryoprotectent obtained to powdered nanosuspension. The lyophilized nanosuspension was characterized for their particle size, polydispersity index (PDI) and zeta potential. The F2 formulation observed particle size of 78.62 nm with PDI 0.223 and zeta potential shows -18.8 mV showed as best formulation. The selected formulation F2 indicated uniform particle size distribution. The surface morphology was characterized through SEM. The optimized formulation was further evaluated for FT-IR, HPTLC, saturation solubility and dissolution rate. The saturation solubility of lyophilized formulation F2 was 3.4 times greater in distilled water compared to pure extract of phyllanthin and 2.12 times increased in phosphate buffer pH 6.8. Entrapment efficacy and drug content of F2 showed 79.22% and 88.41%. The dissolution rate of F2 formulation showed 90.30 % release compared to pure extract powder which followed the first order release kinetics. Hence the lyophilized nanosuspension using high speed homogenization technique resulted in efficient particle size reduction can be successfully employed to improve the solubility and dissolution rate of Phyllanthin with piperine as bioenhancer.

Keywords: Nanosuspension, Phyllanthin, piperine, High speed homogenization, lyophilization.