Abstract

A STUDY ON EFFECT OF PHYLLANTHUS EMBLICA AND AMARANTHUS VIRIDIS PLANT EXTRACTS ON SEED GERMINATION OF GREEN GRAM (VIGNA RADIATA)

Aathiya Vanekar, Suresh Kumar C., Pratibha K. Y., Geetanjali R., Khamer Ferzana Banu and Keshamma E.*

ABSTRACT

The present study was carried out with the main aim to evaluate the effect of treatment of plant extracts of Phyllanthus emblica and Amaranthus viridis at concentration 20%, 40%, 60%, 80% and 100% on seed germination of Green gram (Vigna radiata). Plant extracts were prepared by grinding 200 grams of fresh and healthy leaves in 100 mL of distilled water and these leaf extracts were used to evaluate germination percentage of mung bean seeds. Green gram seeds were soaked for 24 hours in a distilled water. After 24 hours the seeds were treated in the leaf extracts of different concentrations for 2 hours. After 2 hours of seed treatment the seeds were transferred to the petriplates in the following manner- 2 in the centre, 8 in the 1st circle and 15 in the 2nd circle (Standard blotting method). The petriplates were kept at normal day light and at room temperature. Drops of water were added to the plates twice a day and the seed germination was observed for 7 days. Experiments were conducted in the closed petriplates and in triplicates. Germination of seeds was observed on daily basis for 7 days. The mean root length, shoot length, leaf length, wet weight and dry weight were recorded after 7 days. The seeds with full growth of radical and plumule were considered normal and the germination percentages were recorded. Results delineated that there was decrease in mean root length, shoot length and leaf length of Green gram (Vigna radiata) following treatment with extracts of Phyllanthus emblica and Amaranthus viridis at the all the concentrations tested viz. 20%, 40%, 60%, 80%, and 100% as compared to control (0%). Highest inhibition was observed in Green gram seeds treated with leaf extracts of Phyllanthus emblica and Amaranthus viridis at the concentration of 100%. The dry and wet weight of Green gram treated with Phyllanthus emblica and Amaranthus viridis extracts was found to be lesser as compared to control (0%). In conclusion, present study discovers allelopathic interaction was evident between the leguminous plant Vigna radiata and the weed Amaranthus viridis.

Keywords: Phyllanthus emblica, Amaranthus viridis, Seed germination, Vigna radiate.