Abstract

DEVELOPMENT AND VALIDATION OF A NEW ANALYTICAL FORCED DEGRADATION AND STABILITY INDICATING METHOD FOR THE SIMULTANEOUS ESTIMATION OF SATRANIDAZOLE AND OFLOXACIN BY RP HPLC

*Keerthi K., Soumya S., Umarani Y. and Dr. Nasreen Sultana

ABSTRACT

A simple and selective LC method is described for the determination of Satranidazole and Ofloxacin in tablet dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of 50 volumes of Triethylamine buffer,50 volumes of acetonitrile with detection of 238 nm. Linearity was observed in the range 60-140 μg/ml for Satranidazole (r2 =0.999) and 6-14 μg /ml for Ofloxacin (r2 =0.996) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim.The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.

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