Abstract

ICP-MS ANALYSIS, GC-MS IDENTIFICATION OF ANTIMICROBIAL, ANTIOXIDANT, ANTIDIABETIC VOLATILE BIOACTIVE CONSTITUENTS FROM TRIBULUS TERRESTRIS FRUITS

Ali Alrabie*, Nabeel Abdullah Alrabie, Mohammed AlSaeedy, Arwa Al-Adhreai, Inas Al-Qadsy, Ola Basa?ar, Mazahar Farooqui

ABSTRACT

Tribulus terrestris L. belongs to the family Zygophyllaceae. It is known as the devil's thorn. The purpose of this study was to assess several analytical properties of Tribulus terrestris fruits as well as some biological activities such as antimicrobial, antioxidant, and antidiabetic activity of methanol extract in vitro. Tribulus terrestris fruit powder was tested for heavy metals using ICP-MS, and then methanol extract was tested for Gas chromatography Mass Spectrometry (GC-MS). An additional benefit of methanol extract was tested in vitro for antibacterial, antioxidant, and anti-diabetic properties. Cr, Mn, Fe, Ni, and Cu were found to be over the Food and Agriculture Organization/World Health Organization (FAO/WHO) acceptable limits for edible plants by inductively coupled plasma-mass spectrometry (ICP-MS) analysis. Eleven peaks were found in the GC-MS analysis, indicating that the methanolic extract contained eleven distinct phytochemicals. For the first time, Tribulus terrestris fruit has been reported to contain pectolinarigenin, α-patchoulene, and thujopsene. The methanolic extract showed significant antibacterial action against E. coli (MIC 100 µg/mL). Strong antioxidant activity was demonstrated in DPPH assays, as well as a high degree of antidiabetic efficacy. This study gives critical diagnostic traits for the species Tribulus terrestris, which utilized to identify it.

Keywords: acceptable limits for edible plants by inductively coupled plasma-mass spectrometry (ICP-MS) analysis. Eleven peaks were found in the GC-MS analysis, indicating that the methanolic extract contained eleven distinct phytochemicals. For the first time, Trib