Abstract

SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACIN AND METRONIDAZOLE FROM PHARMACEUTICAL DOSAGE FORM BY ABSORBANCE RATIO METHOD

*Pralhad Rege, Kyle Meyers and Neha Kapadia

ABSTRACT

In present study, a successful attempt has been made to develop a simple, sensitive, accurate and economical spectrophotometric method for simultaneous estimation of Norfloxacin and Metronidazole in tablet dosage form. The method is based on Q-analysis (absorbance ratio method). Norfloxacin and Metronidazole shows absorbance maxima at 275 nm and 325 nm in 0.1N sodium hydroxide, respectively. The linearity was obtained in the concentration range 2 μg / mL to 20 μg / mL for Norfloxacin at 275 nm and 2.5 μg / mL to 25 μg / mL for Metronidazole at 325 nm. In given method the concentration and amount of drugs was determined by using ratio of absorbance at iso-absorptive point (290 nm) and at λmax of one of the drugs (275 nm). The results of the analysis have been validated statistically and by assay, recovery studies. Therefore, the proposed validated method can be successfully applied for routine quality control analysis and simultaneous determination of norfloxacin and metronidazole in combined drug formulations.

Keywords: Norfloxacin, metronidazole, absorbance ratio method, spectrophotometric method and validation.