Abstract

HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR ANALYSIS OF AN ANTI-HISTAMINIC FORMULATION

*Vaishnavi B. Akotkar, Pooja G. Sakharkar, A. P. Dewani and Proff. Dr. A.V. Chandewar

ABSTRACT

The measurement of bambuterol hydrochloride and montelukast sodium in combined dosage form using reverse phase high pressure liquid chromatography is described in this research report as being straightforward, specific, accurate, and precise. Reverse phase C8 column (Phenomenex C8, 5, 250mm x 4.6mm) was used to analyse the sample, with methanol, acetonitrile, buffer (Sodium acetate acetic acid) (Ph 6.2), and 1% trichloroacetic acid as the mobile phase. The ratio of these three components was 35:35:30 v/v/v. Utilizing 218 nm UV detection, quantification was accomplished. Bambuterol Hydrochloride and Montelukast sodium were shown to have retention times of 3.259 and 5.007 min, respectively. The linearity for both medications was in the 0.5–10 g/ml range, and the mean accuracies for bambuterol hydrochloride and sodium montelukast, respectively. Since there were no chromatographic interferences from the tablet excipients, the approach was subsequently used to pharmaceutical formulation. When using the common addition approach, the procedure kept its accuracy and precision

Keywords: Bambuterol Hydrochloride, Montelukast sodium, RP-HPLC, Combined Dosage Form.