DEVELOPMENT AND VALIDATION METHOD FOR THE DETERMINATION OF ANTIBIOTIC DRUG BY REVERSE PHASE HPLC TECHNIQUE
*Dhiraj D. Mangam, Pooja G. Sakharkar, A. P. Dewani, C. K. Gadewar and Proff. Dr. A. V. Chandewar
ABSTRACT
Numerous infections are treated with antibiotics. Moxifloxacin is utilised in numerous therapeutic procedures. A method involving an HPLC-diode array detector (DAD) was developed and proven for measuring moxifloxacin in pharmaceutical dose form. Chromatographic analysis was carried out using a Waters 717plus HPLC system, and a Hypersil Gold C18 analytical column was used for separation (250 4.6 mm id, 5 m). It was discovered that the mobile phase containing mixture of 10mM Buffer: Methanol in the ratio of 60:40 v/v pH 4.4 adjusted with ortho phosphoric acid at a flow rate of 1.0 ml/min yielded adequate retention time of about 8 min with sharp symmetrical peak at 294 nm. The strategy was shown to be precise (%RSD), linear (R2 > 0.999), and targeted. Pharmaceutical.
Keywords: HPLC, Moxifloxacin, DAD, Mobile phase, Chromatography etc.
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