Abstract

METHOD DEVELOPMENT AND VALIDATION OF NIMORAZOLE TABLET DOSAGE FORM BY UV SPECTROPHOTOMETRY

A Santosh Kumar Sreevatsav*, N.Mamatha, MD.Fiaz, W.A.Karthikayan, G.Latha

ABSTRACT

A new U.V.spectophotometric method was developed and validated as per ICH guidelines for the quantitative determination of nimorazole. The solvent used is mixture containing acetonitrile:water(50:50).The wavelength corresponding to maximum absorbance of the drug was found to be 302nm. Beer’s law was observed in the concentration range of 5-25μg/ml. The method was validated for several parameters like accuracy, precision, robustness as per ICH guidelines. The values of relative standard deviation and %assay were found to be satisfactory indicating that the proposed method is precise and accurate and hence can be used in the routine analysis of nimorazole in bulk and pharmaceutical formulations.

Keywords: U.V., ICH guidelines, acetonitrile.