Abstract

A REVIEW OF LIQUID CHROMATOGRAPHY HYPHENATED TECHNIQUES AND THEIR APPLICATIONS

Dr. S. Joshna Rani and Chitta Srividya*

ABSTRACT

Chromatography is defined as separating a mixture of components into an individual component through equilibrium distribution between two phases where it is used for both qualitative and quantitative analysis, it is used to separate inorganic and organic compounds that are studied and analysed. The hyphenated technique is a combination /coupling of two analytical techniques with help of a proper interface. To separate components within a sample substance to identify each component it. Over the last two decades, impressive advancements in hyphenated analytical techniques have greatly widened their applicability in the investigation of biomaterials, particularly natural goods. Hyphenation technique gives fast, accurate and also has better reproducibility in results. The hyphenated technique gives the structural information leading to the identification of compounds. The hyphenated technique involves numerous fields such as forensic science, environment, biotechnology, geography, pharmaceutical, etc. are presented with suitable examples for diverse purposes. This review provides information on analytical methods mainly includes LC-MS, LC-FTIR, and LC-NMR.

Keywords: Chromatography, Hyphenated technique, LC-MS, LC-FTIR, LC-NMR.