IN-VITRO SCREENING OF PHYLLANTHUS URINERIA FOR ANTIMICROBIAL ACTIVITY USING DISK DIFFUSION METHOD
Boby Disoriya*, Arvind Singh Jadon and Poonam Bhadauriya
ABSTRACT
Leaves with Arial parts and fruits of plant Phyllanthus urinaria were collected and dried under shade. Powdered plant materials were subjected for extraction, after the extraction, phrmacognostical evaluation was done. Extracts were tested for phytochemical and found leaf extract contains tennins, flavonoids, glycosides, alkaloids, saponins and phenolics and fruit extract contains flavonoids, glycosides, saponins, phenolics, anthraquinones and alkaloids. Gradual increasing percentage inhibition with increasing concentration was observed as antioxidant activity by DPPH assay. Extracts of Phyllanthus urinaria was evaluated against Gram positive (B. subtilis)
and Gram negative bacteria (E. coli). Ethalolic extract of leaf was effective against E. coli but minute active against B. subtilis bacteria, Aqueous extract of leaf moderately effective against both Gram positive and Gram negative bacteria. Ethanolic extract of Seed was moderately effective against both Gram positive and Gram negative bacteria. Aqueous extract of seeds was moderately effective against both Gram positive and Gram negative bacteria. Extracts of Phyllanthus urinaria was evaluated against A. niger and C. albicans. Ethalolic extract of leaf was effective against A. niger but moderately effective against C. albicans, Aqueous extract of leaf less effective against both Candida albicans and A. Niger. Ethanolic extract of Seed was moderately effective against Candida albicans but less effective against A. Niger. Aqueous extract of seeds was moderately effective against Candida albicans but less effective against A. Niger.
Keywords: Phyllanthus urinaria, antimicrobial, Gram positive, antioxidant, DPPH assay, phytochemical.
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