Abstract

INVITRO ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF LEAF EXTRACT OF MYRICA NAGI LINN

Nadeem Akram* and V. Venkateswaran

ABSTRACT

Introduction: The main objective of the present study is to evaluate the antioxidant and antimicrobial activity of the leaf extracts of Myrica nagi considering its future industrial requirement and commercial utility as nutraceuticals. Matirials and Methods: Invitro Antioxidant activities of Ethanolic extracts were evaluated by DPPH radical scavenging assay ABTS radical cation scavenging assay, Lipid peroxidation (LPO) assay and Pharmacognostic and Phytochemical evaluation by different methods like ash test, reagent test. Antimicrobial activity also done by various methods like Disc diffusion methods etc. Result: Anti oxidant activity of leaf extracts of Myrica nagi- EEW extract exhibited potent antioxidant activity with a IC50 value of 68 μg/ml among all the test samples under DPPH method. The standard antioxidants ascorbic acid and rutin (controls) exhibited IC50 values of 56 and 53 μg/ml respectively. All the samples tested using hydrogen peroxide method, EEW extract exhibited moderate activity with a IC50 value 270.7 μg/ml. When compared to all the test samples under hydrogen peroxide method. The standard antioxidants ascorbic acid and rutin (controls) exhibited IC50 values of 172.36 and 46.15 μg/ml respectively. All the samples tested using Nitric oxide radical inhibition method EEW extract exhibited potent activity with a IC50 value 140 μg/ml. The known standard antioxidants ascorbic acid and rutin (controls) exhibited IC50 values of 30 and 185.5 μg/ml respectively. EEW extract exhibited moderate antioxidant activity under lipid peroxidation method. All the other test samples did not show any significant antioxidant activity. The known standard antioxidants α – tocopherol (control) exhibited IC50 value of 65 μg/ml respectively. Conclusion: The present study is to screen the antioxidant and anti microbial activity of the leaf extract of Myrica naga with an objective of utilizing the extract as a neutraceuticals in future. The result of the study confirms the antioxidant property of the leaf extract of Myrica naga by Nitric oxide radical inhibition activity method. The extract also showed potent antioxidant activity under DPPH, Lipid per oxidation, ABTS and Hydrogen peroxide method. Which confirms that scavenging ability of the extract towards the stable radical DPPH, Hydrogen peroxide radicals, ABTS method (2,2’ – azinobis, 3 – ethyl benzo thiazoline– 6 – sulfonic acid). Although these results were obtained at a comparatively higher dose, they are encouraging for further in vivo studies with an aim of obtaining clinically useful results.

Keywords: Myrica nagi, Anti oxidant, Anti microbial, Anti inflammatory.