Abstract

CURCUMIN LOADED CHITOSAN NANOPARTICLES FORMULATION AND OPTIMIZATION BY IN VITRO AND EX VIVO EVALUATION.

Shravan L Nargund, Dr. C. S. R. Lakshmi, Shachindra L. Nargund,
Dr. L. V. G. Nargund and Abdul Naim*

ABSTRACT

The following study is designed to formulate, optimize, and evaluate curcumin, loaded into chitosan nanoparticles aiming for the novel delivery of the drug to the site of action. Ionic gelation method was considered in the preparation of nano scale molecules. Design expert software v10 was used with 24 full factorial design in optimizing the concentration of constituents present in the preparation of nanoparticles. In which Chitosan concentration, Acetic acid concentration, Sodium tripolyphosphate (TPP) concentration and pH were the factors and the results were particle size, encapsulation efficiency (%EE), zeta potential, polydispersity index (PDI) and Inhibition concentration of 50% on MCF7 cell lines (IC50) were taken into consideration. A set of 16 trials were processed and optimization was carried out. The factors showed significant effect on particle size, zeta potential and IC50 values. The optimized formulation contained 2mg/ml chitosan concentration, 7mg/ml sodium TPP concentration, 7% acetic acid concentration and the pH was maintained at 6. The results were 630.92nm particle size, 0.685 PDI, percentage encapsulation efficiency of 97%, IC 50 at 136.85μM and -8.175 zeta potential. The desirability of the outcome was 0.861.

Keywords: .