Abstract

REDUCING THE ALBENDAZOLE DOSE BY PAPAIN USE FOR ANTHELMINTIC FAST DISSOLVING TABLETS

Deep Chandra*, Geetika Tripathi and Manish Mishra

ABSTRACT

A variety of products Albendazole and Papain are separately available in the market but in no product, both of these agents used together to encounter the endo-parasites. Therefore, the present work was aimed to formulate fast dissolving tablets where albendazole dose was reduced and placed by papain for similar effects of BENDI tablets (an established product of albendazole marketed by Mankind). In the physicochemical evaluation, melting point & FTIR spectral values were within the range of literature values whereas papain enzymatic activity was found to be 0.284μg ± 0.25 of p-nitroaniline per minute at 25°C. After in-vitro adsorption study of drug on primojel, kollidon & croscarmellose the later was selected to prepare SSD with highest percent drug adsorbed on its surface as 86.45 % ± 0.5 w/w in 72 hours. Out of six SSDs (S1-S6), S4 with 1:2 drug to polymer ratio showed highest enhancement in drug solubility (524.94 ± 0.18 %) and also showed satisfactory flow properties. This was used to prepared eleven (F1 to F11) fast-dissolving tablets according to a tailored experimental designed model. Hardness, weight uniformity, friability, drug content & disintegration time were recorded for effectiveness as tablet. % CDR was in the range of 87.45±0.45 to 93.42±0.72 % for albendazole and 89.52±0.34 to 94.25±0.98 % for papain. In-vitro egg hatching (EH) test and larval motility (LM) test was conducted with Bendi as the positive control and phosphate buffer solution pH 7.4 as negative control. Mean values were calculated at (P < 0.05) and by using one-way ANOVA at 5 % significance level. After which, F9 was selected as it inhibited EH > 97% and LM > 95% which proved its efficacy against nematodes that was equivalent to the positive control. But at the same time, containing lesser albendazole dose (320mg) as compared to Bendi. Rest compensatory effect against nematodes was produced

Keywords: by 60mg Papain dose. In this way, use of papain reduced the overall 400mg dose of albendazole to 320mg that is 20% reduction in the original marketed dose of albendazole. This will reduce the drug side effects to a great extent in the long-term therapies