Abstract

EVALUATION OF WOUND HEALING POTENTIAL OF MORINGA SEED OIL IN RAT USING EXCISION MODEL

Anand Sharma* and Sandeep Jain

ABSTRACT

The present investigation was to evaluate the wound healing action of Moringa seed oil in rats by formulating as an ointment. The seed oil was extracted using solvent extraction method. The saponification value, iodine value, pH, density, refractive index and free fatty acids were determined. The saponification value, iodine value and free fatty acid of the extracted Moringa seed oil were calculated. The density, pH and refractive index of extracted moringa seed oil were also found. The acute toxicity study reveals a LD50 of more than 2000 mg/Kg for Moringa seed oil. The Moringa seed oil was evaluated for the in vivo wound healing effect by the excision model. The topical application of 5% w/w of the Moringa seed oil resulted in an enhanced and statistically significant (p < 0.001) wound healing activity in vivo. The Moringa oil ointment exhibited 64.93 ± 5.86% contraction of wound on the 20th day whereas only 48.51 ± 5.46% contraction of wound was found in the control animals.

Keywords: Moringa seed, solvent extraction, topical, wound healing, excision model.