Abstract

EVALUATION OF COW-DUNG DUMPING AREA SOIL FOR ISOLATION OF ANTIBIOTIC PRODUCING BACTERIA

Chandel Ekta, Deshwali Ankur, Verma Mamta*

ABSTRACT

The need for new antibiotics has emerged with the increase in the pace of the emergence of drug-resistant pathogens. The Discovery of a new antibiotic from nature is becoming an area of interest for drug discovery. The present study had been done to evaluate different sources of soil for the isolation of antibiotic-producing bacteria. The soil samples were collected from different locations of Ujjain and subjected to primary and secondary screening for analysis of antibiotic-producing bacteria. The primary and secondary screening was performed by crowed plate technique and antimicrobial assay. Sample 1 to 5 were collected from crop field, dumping area, underneath of flowering plant, Mahakaal van kshetra and dumping area of cow dung respectively. In primary screening total of 38 bacterial isolated were found which were showing antimicrobial activity among them in samples 1, 7, sample 2, 7, sample 3, 4, sample 4, 6, and from sample 5, 15 antibiotic-producing bacteria were found and isolated. MJEC201538 has the best activity among all against P. aeruginosa ZOI 23mm. While MJEC201535 ZOI of 30mm against E.coli and MJEC201536 having ZOI of 30 mm against E.coli and S.aureus. The bacterial isolate MJEC201538 was showing the best antimicrobial activity and was subjected to identification using Bergey's Manual and reported to be Bacillus subtilis. From the present study, it has been concluded that the soil of the dumping area of cow dung can be the rich source for the isolation of antibiotic-producing bacteria.

Keywords: Bacillus subtilis, Antibiotics, Soil bacteria, Cow dung, Ujjain.