Abstract

DEVELOPMENT AND VALIDATION OF DISSOLUTION METHOD FOR ZIDOVUDINE TABLETS USING UV VISIBLE SPECTROPHOTOMETRY

Santosh Gandhi* and Vinod Gaikwad

ABSTRACT

The current study outlines a simple, accurate, quick, precise, and sensitive UV spectrophotometric absorption method for determining Zidovudine in bulk and tablet dosage form as well as dissolution media. As a solvent, distilled water was employed with detection at 265 nm in the concentration range of 5-30 μg/mL with linear equation y=0.0303x+0.0259 and r2 of 0.984. The mean percentage drug content for Zidovudine in commercial formulation was found to be 100.418 ± 1.719. The method found to be sensitive with limit of detection and limit of quantification 1.320 μg/mL and 3.886 μg/mL, respectively. The water was used as dissolution media with more than 95 percent dissolution observed at 30th minute.

Keywords: Zidovudine, UV-visible spectrophotometry, Dissolution, Validation.