Abstract

SUSCEPTIBILITY PATTERN OF STAPHYLOCOCCUS AUREUS ISOLATED FROM HEALTHY COMMUNITY INDIVIDUALS TO BUCHHOLZIA CORIACEA ENGL. (FAM: CAPPARACEAE) LEAF EXTRACT

Clement Oliseloke Anie* and Olukayode Temitope Egbon

ABSTRACT

Objective: The in vitro antimicrobial activity of crude extract of leaf extracts of Buchholzia coriacea against Methicillin-resistant Staphylococcus aureus. Methods: The aqueous and methanolic extracts Buchholzia coriacea were investigated for their phytochemical constituents; while the MRSA was identified using oxacilin sensitivity disk. Antimicrobial activity was evaluated using seeded plate method. Phytochemical screening revealed the presence of tannin, anthroquinone, glycosides, steroids, terpenoid, alkaloids. The minimum inhibitory concentration of methanol and aqueous leaf extracts of B. coriacea on selected MRSA isolates. Results: The results show that every isolates had growth at 12.5 mg/ml concentration but none of them had at 200 mg/ml – 50 mg/ml concentrations. At 25 mg/ml concentration isolate 3 had growth. MIC of the methanol leaf extract of B. coriacea against isolates 1, 2, 4 and 5, was 25mg/ml, while that for isolate 3 MIC is 50 mg/ml. MIC of aqueous leaf extract of B. coriacea on selected MRSA isolates. MIC of aqueous leaf extract of B. coriacea on selected MRSA isolates shows that all the isolates were able to grow at 25 mg/ml and 12.5 mg/ml concentrations. Contrarily, none had growth at 200 mg/ml and 100mg/ml concentrations. However at 50 mg/ml concentration, isolate 3 had growth, while other isolates had no growth 50 mg/ml. Therefore, the aqueous extract had minimum inhibitory concentration for isolate 3 at 100 mg/ml as against 50 mg/ml for isolates 1, 2, 4 and 5. The aqueous extract had higher MIC concentrations than the methanol leaf extract in agreement with earlier reported. Conclusion: Further study to be carried out to fractionate the leaf extracts which could enhance the bacterial activity.

Keywords: Methanol, Buchholzia coriacea, Aqueous extract.