Abstract

COMPARATIVE STUDY OF HPLC AND UPLC AN OVERVIEW

Pooja S. Kakade* and Vikas Kandekar

ABSTRACT

The Liquid Chromatographic procedures HPLC and UPLC are both used to separate the components of a substance. To understand the differences between HPLC and UPLC, we must first understand HPLC. This is due to the fact that UPLC is a variant of HPLC. Recent advances in pharmaceutical analysis have made chromatographic media with a 1.7 m particle size, as well as a liquid handling system capable of operating such columns at significantly higher pressures, available. Ultra performance liquid chromatography (UPLC), which uses sub 2 micron particles and extremely high pressure (up to 100 MPa in a UPLC system), has shown to increase method sensitivity, resolution, and speed when compared to traditional HPLC. When compared to chromatographic systems using 5 m size particle loaded analytical columns, the UPLC system can reduce analysis time by up to nine times. The analysis time was reduced by nearly three times when compared to 3m particle packed analytical columns. The current review paper distinguishes between HPLC and UPLC, as well as analytical method validation, applications, and HPLC and UPLC advantages and disadvantages.

Keywords: Ultra performance liquid chromatography, High performance liquid chromatography, sensitivity, BEH technology, HSS Technology.