Abstract

A REVIEW ON PROTEOMIC ANALYSIS

Panthagani Sharon*, D. Prasanna, Dr. K. Padmalatha

ABSTRACT

Proteomics is a major component of protein research, especially its function and structure. Proteomics involves the use of technology to identify and quantify the total amount of protein present in a cell, tissue, or organism. Add other “omics” technologies such as genomic and transcriptomics to explain the protein identity of an organism, as well as to identify the structure and functions of a particular protein. Proteomics-based technologies are used in a variety of ways in different research settings such as the discovery of different diagnostic criteria, people who will develop vaccines, understanding pathogenicity patterns, modification of patterns that respond to different signals and interpretation of protein mechanisms that work in various diseases. Proteomics is really complex because it involves the analysis and classification of all genome protein signatures. Techniques that include 2-D gel electrophoresis, mass spectroscopy with LC-MS and MALDI-TOF / MS etc., play an important role in analysing protein novels and their role in disease retention and treatment of widely used intermediate proteomics amine. However, the use of proteomics resources which includes equipment software, database and the need for skilled workers greatly increases costs, thus reducing their widespread use especially in developing countries. In addition, the proteome is highly robust due to the complex control systems that control the levels of protein production. In the current review it focuses on the different types of protein analysis techniques demonstrated and also focuses on the effective detection of proteomics in the medical, agricultural and food fields.

Keywords: Proteomics; 2-D electrophoresis; MALDI-TOF/MS; SILAC; Matrix; Applications.