Abstract

FORMULATION DEVELOPMENT AND EVALUATION OF HEPATOPROTECTIVE ORAL DRUG DELIVERY SYSTEM FROM CYATHEA GIGANTEA

Baijnath Singraul*, Aditya Tiwari and Dr. Nitendra Sahu

ABSTRACT

Hepatoprotective oral drug delivery system from cyathea gigantea was developed. The powdered plant material contains active chemical constituents were extractd. % Yield of Pet. ether and Hydroalcoholic extract of Cyathea gigantea were found 2.58 and 5.76% respectively. Total phenol content was found 0.756mg/100mg, gallic acid equivalent in hydroalcoholic extract. The concentration of alkaloid in crude extract 0.632mg/100mg Atropine equivalent in dry sample. Different formulation of phospholipids complex of hydroalcoholic extract of Cyathea gigantea were prepared different ratio of phospholipids and cholesterol and evaluated for Microscopic observation of prepared Phytosome, Drug Excipient compatibility study by FT-IR, Entrapment efficiency, Particle size and size distribution, Transmission Electron Microscopy, In-vitro drug release studies and Stability studies. The entrapment efficiency of all the prepared formulations were determined. The entrapment efficiency of the phospholipids complex was found in the range of 45.65±0.25 to 69.98±0.14%. Particle size of all formulations found within range 245.23±0.63-355.25±0.32nm. Concentration of lipid has shows significant impact on size of phospholipids complex. Formulation F4 was found best one which is further evaluated for drug release study, transmission electron microscopy (TEM), and stability studies. Higuchi was maximum 0.993 hence indicating drug release from formulations was found to follow Higuchi release kinetics. Results of stability studies clearly indicates that optimized batches of phospholipids complex were stable over the chosen temperature and humidity conditions up to 3 months as were found no significant variation in physical appearance and % drug content. It can be concluded that the phospholipids complex containing Smilax china can provide a convenient and safe alternative to dosage form. Formulation F4 was also tested for hepatoprotective activity against Hep G2 and co-culture with THP1 by MTT assay. Prepared Phospholipids complex F4 showed the IC50 value about 100μg/ml.

Keywords: Hepatoprotective, cyathea gigantean, phospholipids complex, Smilax china, Phytosome.