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Abstract

DANIO RERIO EMBRYO AS A MODEL FOR STUDY ABORTIFACIENT EFFECTS USING ANANAS COMOSUS

Abhinava J V, Srinivas Raju, Guruprasad R*

ABSTRACT

Danio rerio is one of the best model organisms to study various kinds of disorders. This study was conducted by treating Danio rerio embryos with a well-known plant having abortifacient activity like Ananas comosus. The ethanol and chloroform extract were subjected to the embryos at different post fertilization periods and this includes 1cell stage, blastula, gastrula and organogenesis. The effects of the different concentrations were studied at different time intervals. From the statistical analysis for time with concentration, the blastula stage embryos show the most significant effect P (F≥0.8753) = 0.05. As embryos develop, they get resistance against both extracts and the survival rate increases. Prolonged exposure to the embryos shows death in all stages. This experiment demonstrates that Danio rerio is a suitable model organism for the study of abortifacient activity.

Keywords: abortifacient, Danio rerio, Ananas comosus, model organism, embryogenesis.


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