HEAVY METAL FREE POTABLE WATER: APPLICATION OF BIO-EMULSIFIER PRODUCED BY ACINETOBACTER SPECIES ISOLATED FROM SOIL IN LEAD REMOVAL
Patil Sayujya Pritamraj and Vemula Anvesh Narshimlu*
ABSTRACT
Lead intoxication cases even if kept unattended are causing great havoc in health of exposed people lives. Lead successfully enters human food chain by numerous paths, which is somehow explained by bio-magnification of lead in food chain. Lead has access through means of different compounds and major source are the PVC (poly vinyl chloride) pipes that uses Lead Nitrate and Lead Acetate a stabilizer. Poisoning effects could range from abdominal pain to learning disability. Acinetobacter species is a ubiquitous in nature and have earlier proven to exhibit bio-emulsifying properties which can have major impact in removal of heavy metals like Lead (Pb) from soil as a part of bioremediation. Isolating these species from soil is a cherry pie when site selection is prominently with high vicinity of hydrocarbon compounds, is performed with effective enrichment using Dijkshoorn’s enrichment media in aerobic condition. Selective
isolating media (for instance: Herellea Agar) is used to isolate and subject them for bio-emulsifier production by maintaining restricted carbon source as 2% (v/v) Olive oil in MSM which is mostly recommended after sunflower oil. Evaluate the product with distinct tests like (i) Oil spread assay and (ii) Emulsification index (E24). Genuine product needs extraction and purification by various means of solvents. Evaluation of purified product’s ability to reduce the concentration of Lead Nitrate from potable water is checked and compared with control kept against without addition of product.
Keywords: Lead Removal, Acinetobacter species, Bio-emulsifier, Olive oil, Dijkshoorn’s media, Aerobic, Hydrocarbons, Bioremediation.
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