Abstract

PHYTOCHEMICAL EVALUATION OF FERMENTED FRUIT PULP EXTRACT OF ZIZIPHUS MAURITIANA LAM. BY USING HRLC-MS TECHNIQUE

*Jadhao Anand and Shaikh Farah T.

ABSTRACT

The aim of present study was to characterize Ziziphus mauritiana fruit pulp for the presence of biologically active phytochemical constituents using traditional fermentation technique and High Resolution Liquid Chromatography - Mass Spectroscopy (HRLC-MS). Mass spectra of compounds found in the extract was matched with Metlin database library, results confirmed the presence of therapeutically potent compounds in Z. mauritiana fruit extract. Metabolites analysis by ESI-Q-TOF-MS revealed the presence of 10 major abundant compounds viz, 6-Methyl-2-methylene-6-octene-1,3,8-trio, 6alpha-Carissanol, 4,11,13,15-Tetrahydroridentin B, Atractylone, 4-Acetoxy-2-hexyltetrahydrofuran, methyl (2E,6E,10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate, Humulene-8-hydroperoxide, 6- Oxabicyclo[3.1.0] hexane-2-undecanoic acid methyl ester, Zerumbone oxide, 3-dehydroecdysone, and Cavipetin D. The medicinal values of fruit may be due to existence of such important metabolites in pulp.

Keywords: Ziziphus mauritiana, fermentation, HRLC-MS, Phytochemicals.