Abstract

BIOSYNTHESIS OF GOLD NANOPARTICLES USING PIPER NIGURM AND THEIR ANTIBACTERIAL ACTIVITY

R. Devika, S. Elumalai*

ABSTRACT

In this work, environmentally benign process of gold nanoparticles (Au-NPs) have developed using Piper nigrum leaf extract has been attempted. The bio reduction of Au+ ions in solution was monitored using UV-visible spectroscopy from zero reading and double distilled water was used as blank. The samples were withdrawn at various time intervals and the absorbance was measured. The gold nanoparticles synthesized using extracts of samples was confirmed by color changes and was characterized by UV-visible spectrophotometer; the UVvisible spectra showed a broad peak located at 535 nm for gold nanoparticles. The Scanning Electron Microscopy (SEM) analysis shows the surface morphology of Au-NPs. Thus gold nanoparticles has great potential as antimicrobial compound against pathogenic microorganisms.

Keywords: Gold nanoparticles, Piper nigrum, Antimicrobial Activity, Electron microscopes and Spectroscopic studies.