Abstract

EFFECT OF ALCOHOLIC EXTRACT OF TECTONA GRANDIS LINN. HEART WOOD AGAINST OXIDATIVE STRESS AND DIABETIC CONDITIONS

Anoop Agnihotri*, Vijender Singh¹

RV Northland Institute, Dadari, Greater Noida, U.P., India
¹B.B.S. College of Pharmacy, Greater Noida, U.P., India.

ABSTRACT

Tectona grandis Linn. f. (Family - Verbenaceae); is a large deciduous tree found in peninsular regions in India. Heart wood of this plant is mentioned in Ayurved (Indian Traditional System of Medicine) for the treatment of diabetes. Alcoholic extract of T. grandis heart wood (TGHW) was evaluated for anti-hyperglycemic effect in alloxan induced diabetic rats. Blood glucose level, serum cholesterol, triglycerides, serum albumin, total protein, creatinine levels and body weight were studied for three weeks. Antioxidant potential in DPPH, nitric oxide and hydroxyl radical induced in vitro assay methods was evaluated. Acute toxicity studies were carried out to establish the safety of the drugs as per OECD guidelines. There was a significant decrease in elevated blood glucose level (p<0.01) in diabetic animals treated with TGHW. Significant reduction in serum creatinine (p<0.05), serum triglyceride, serum cholesterol (p<0.01) and increase in serum albumin, total protein and body weight (p<0.01) was observed when compared with diabetic control group. Plant extract also showed significant antioxidant activity in DPPH, nitric oxide and hydroxyl radical induced in vitro assay methods. Acute toxicity studies showed no signs of toxicity up to a dose level of 2000 mg/po. TGHW was found more effective at a higher dose of 500 mg/kg. Recovery in the blood glucose level and other biochemical parameters signifies the hypoglycemic and nephroprotective effect of Tectona grandis heart wood in the treatment of diabetes and related disorders..

Keywords: Antidiabetic, Oxidative stress, Renal complications, Tectona grandis, Nephroprotective.