Abstract

ENHANCING CYTOTOXICITY ACTIVITY OF CURCUMIN-ENTRAPPED TDDS IN SKIN CANCER: USING HYDROPHILIC AND HYDROPHOBIC POLYMERS

Sandhiya V.*, Ubaidulla U., Praveen Kumar M., Prasanth Raj CN, Nirmal P., Mano A. and Suhaib I.

ABSTRACT

Aim: This study aims to fabricate and evaluate curcumin loaded transdermal patches against tumor cells. Method: Curcumin loaded transdermal patches were formulated by solvent casting method and evaluated for pre-formulation and post-formulation studies, such as FTIR, drug content, entrapment, Folding endurance, weight and thickness of patches, moisture content, tensile strength, in-vitro bioavailability studies, kinetic study and in-vitro cell line studies using MTT-assay. Result: The pre-formulation studies revealed there was no interaction between drug and polymer so it can be chosen for TDDS. Among nine formulations, F9 shown good diffusion rate, and the percentage of drug release was found to be 96.7%, which might be due to presence of PVA in higher concentration. This could increase the film forming property on skin and act as a good adhesive agent so thereby it can release a drug for sustained period of time. Curcumin loaded patch shown higher rate of cytotoxicity activity compare to plain drug, which was analysed by using MTT-assay. Conclusion: From the above findings it was concluded that the prepared patches has good anticancer activity compare to plain drug so this has been a promising lead molecule to act against cancer cells and it was achieved by TDDS. Further we planned to do in-vivo studies to know the release profile better through TDDS.

Keywords: Curcumin, Transdermal patch, Anti-cancer, MTT-assay.