Abstract

A REVIEW: DIFFERENT SPECTROSCOPIC AND CHROMATOGRAPHIC METHODS FOR ANALYSIS OF DRUG

Rushikesh Natu*, Santosh Waghamare, Hemant Kambale and Yojana Kunjir

ABSTRACT

Phytopharmaceuticals are pharmaceutical products composed of natural compounds derived from botanicals instead of chemicals. As phytopharmaceuticals are complex mixture of many components, therefore it is not easy to do analysis of each component. Thus for proper analysis it is very important to understand chemistry of phytochemicals present in herbal products. Based on its characteristics isolation, structural identification and quantification need to be planned accordingly. Hence, first need to look at chromatographic techniques which isolate various types of components. Once the components are separated they should be analysed by spectroscopic methods for identification and quantification. The review highlights various spectroscopic techniques such as UV-Visible, Infrared, Nuclear magnetic resonance and Mass spectroscopy which are applied for analysis of phytochemicals present in herbal formulations.

Keywords: Phytopharmaceuticals, spectroscopic analysis, UV-Visible spectroscopy, IR spectroscopy, FTIR, NMR spectroscopy, Mass spectroscopy.