Abstract

NEUROPROTECTIVE ACTIVITY OF STACHYTRAPHETA INDICA ON ROTENONE INDUCED PARKINSON’S DISEASE IN ZEBRA FISH

Jayanth A, Muralidhar S.Talkad*

ABSTRACT

Parkinson’s disease (PD) is quiet peculiar owing to its sudden and rapid occurrence. Off late, several environmental toxins have been known to trigger PD. Therefore, such Neurotoxin-based models (particularly MPTP / Rotenone) have become preferred and important models in studying the degeneration of cholinergic and dopaminergic neurons. In this study we establish Neuroprotective activity of Stachytarpheta indica methanolic extract on Rotenoneinduced Parkinsonion condition in Zebra fish.The effect of this plant extract has been studied in comparison with L-DOPA (drug used in treatment of PD). The prime focus of the study revolves around two key enzymes namely Acetylcholine esterase[E.C 3.1.1.7] and Mitochondrial succinate dehydrogenase [EC 1.3.5.1] which are necessary to monitor neuro degeneration. In additionGSH, SGOT and SGPT were performed to assess the safety and efficacy of the plant extract on Zebra fish. The S.indica plant extract was found to control and reduce the increased activity of Acetyl choline esterase and re-establish the mitochondrial succinate dehydrogenase activity there by reducing neuro degeneration. The reduced neurodegeneration in the extract treated group was confirmed from histopathological studies of the Zebra fish.

Keywords: Neuroprotective activity, Parkinson´s disease (PD), Rotenone, Flavonoids, Acetylcholine esterase, Mitochondrial succinate dehydrogenase, Stachytarpheta indica.