Abstract

A REVIEW ON METHOD DEVELOPMENT AND VALIDATION OF METHOTREXATE BY DIFFERENT ANALYTICAL METHODS

Samatha Ch* and Aleti Raja Reddy

ABSTRACT

Methotrexate (MTX) is the first line drug for the treatment of a number of rheumatic and non- rheumatic disorders. It is currently used as an anchor disease, modifying anti-rheumatic drug in the treatment of rheumatoid arthritis (RA). Despite the development of numerous new targeted therapies, MTX remains the backbone of RA therapy due to its potent efficacy and tolerability. There has been also a growing interest in the use of MTX in the treatment of chronic viral mediated arthritis. Many viruses-including old world alphaviruses, Parvovirus B19, Hepatitis B/C virus, and Human immunodeficiency virus have been associated with arthitogenic diseases and reminiscent of RA. MTX may provide benefits although with the potential risk of attenuating patients’ immune surveillance capacities. This review provides the pharmacokinetic, pharmacodynamic and mechanism of action of the METHOTREXATE, which also includes the review on pharmaceutical analytical techniques like High performance liquid chromatography, Reverse phase-High performance liquid chromatography, Bio- Analytical techniques, Spectrophotometry with the bulk and pharmaceutical dosage forms used for determination and validation.

Keywords: Anticancer, Methotrexate, HPLC Method, Spectrophotometric Method, Bio-Analytical, LC- MS Method.