Abstract

A REVIEW OF ANALYTICAL METHODS FOR DETERMINATION AND ESTIMATION OF MELATONIN BY USING DIFFERENT ANALYTICAL METHODS

E. Ratna Sri and Sreelatha Gangu*

ABSTRACT

Melatonin is a hormone primarily released by the pineal glandat night, and has long been associated with control of the sleep–wake cycle. As a dietary supplement, it is often used for the short-term treatment of insomnia, such as from jet lag or shift work, and is typically taken orally. Evidence of its benefit for this use, however, is not strong. A 2017 review found that sleep onset occurred six minutes faster with use, but found no change in total time asleep The melatonin receptor agonist medication ramelteon may work as well as melatonin supplements, at greater cost but with different adverse effects, for some sleep conditions. It often referred to as the sleep hormone, is a central part of the body's sleep-wake cycle. Its production increases with evening darkness, promoting healthy sleep and helping to orient our circadian rhythm. melatonin therapy will also play a beneficial role in supporting other physiological functions that involve melatonin, including the entrainment and coordination of various biological rhythms. However, the benefits of melatonin treatment and the suppression of unwanted effects can be realized only if the pineal hormone is administered with an appropriate dose and at an appropriate circadian time. Some analytical methods and determination and validation of melatonin are like LC-MS/MS, SPECTROSCOPIC METHODS, HPLC-MS-MS, FLOURIMETRIC METHOD.

Keywords: Melatonin, LC-MS/MS, Spectroscopic methods, HPLC-MS-MS, Flourimetric method.