Abstract

COMPARATIVE EVALUATION OF ANTIOXIDANT POTENTIAL OF EZENUS AND OTHER HERBAL PRODUCTS: AN IN-VITRO STUDY

Manu Chaudhary and Vijay Naithani*

ABSTRACT

The aim of this investigation was to evaluate and compare the antioxidant potential of a patented poly herbal sugar free candy Ezenus with twelve other commercial products. Ezenus candy has Andrographolides, Polyphenols, Flavonoids, Vitamin C etc in its ingredients. Ezenus and different commercial products (MCF-1 to MCF-12) were checked for various parameters like metal chelating activity, Catalase activity, reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidase and Xanthine oxidase activity. Amongst the compared groups Ezenus showed the highest metal chelation with minimum IC50 at 730 ± 35 μg/ml which is 2.6 times more potent than MCF-1 where lowest chelating activity was recorded (IC50 1923 ± 115 μg/ml). Catalytic activity is comparable for Ezenus MCF-3 and MCF-11 (1.5±0.05 μMH2O2/min/mg protein). followed by other products. Reduced glutathione content is comparable in Ezenus , MCF-4 and MCF-5 (1.9±0.06 GSH nmol/mg/protein) followed by other products. Ezenus showed the highest oxidized glutathione content (2.9±0.13 GSSH nmol/mg/protein) followed by MCF-12 which is 13% lower and MCF-11 which is 34% lower. The maximum activity of glutathione peroxidase is found to be present in Ezenus (1.1±0.07 nmol/min/mg protein) followed by MCF-6(9% lower) MCF-3 and MCF-12 which are 18% lower than Ezenus. Maximum Xanthine oxidase inhibitory activity was seen in Ezenus and MCF-4 with minimum IC50 of 110 μg/mL, followed by MCF-11, MCF-5 and MCF-12. From these observations it is concluded that out of 13 products compared for antioxidant potential, Ezenus scores in all of these parameters. MCF-12 is observed to be the second best product.

Keywords: Ezenus, detoxification, metal chelation, reduced glutathione, Xanthine oxidase