SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUSLY ESTIMATION OF ABACAVIR AND LAMIVUDINE IN COMBINED DOSAGE FORM
Divyanshu Sharma*, P. K. Dubey and Archana Tiwari
ABSTRACT
A Simple, precise, accurate, economical and analytically reproducible method developed and validated for estimation of Abacavir and Lamivudine in combined dosage form and in pure drug by simultaneous equation method or vierodt’s method. The stock and standard test solutions were prepared 50% methanol followed by further dilutions by 50% methanol, In proposed simultaneous equation method, for the estimation of Abacavir and Lamivudine selected ʎmax were 289nm and 270nm respectively. Linearity was found by both the drugs in concentration range of 5-25 μg/ml. The results of analysis
have been statistically validated by recovery studies also. Thus the proposed study provides magnificent method for the estimation of both drugs in combined dosage form (Tablet).
Keywords: Simultaneous estimation, Abacavir, lamivudine, spectroscopy.
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