Abstract

SIMULTANEOUS ESTIMATION OF PARACETAMOL AND N-ACETYL CYCTEINE BY RP-HPLC

Sravyasudha* and S. Shoba Rani

ABSTRACT

For the measurement and quantification of N-Acetyl Cysteine and Paracetamol, a rapid, sensitive, and reliable RP-HPLC technique using a Waters HPLC System with PDA detection was designed and validated. Chromatography was carried out on an Inertsil -ODS C18 (250 x 4.6 mm, 5) column with a flow rate of 1.0 ml/min and an effluent of 257 nm using filtered and mixed Degassed Methanol: Acetonitrile (30:70) as a mobile phase. N-Acetyl Cysteine retention periods are 3.304 minutes while Paracetamol retention times are 5.828 minutes.

Keywords: Method Development, Validation, N-Acetyl Cysteine, Paracetamol, RP-HPLC.