Abstract

ASSESSMENT OF ANTI OXIDANT AND ANTI DIABETIC ACTIVITY OF MORUS ALBA ROOTS IN ALBINO WISTAR RATS

C. Bhuvaneswara Rao*, Basini Jyothi and Kothapalli Bannoth Chandra Sekhar

ABSTRACT

To evaluate the antioxidant and antidiabetic activity of methanolic extract of roots of Morus alba L. (Moraceae) MEMA in alloxan induced diabetic rats. The (MEMA) have been proven experimentally to possess antioxidant activity in invitro methods. MEMA showed effective response on 2, 2-Diphenyl-1-picryl-hydrazyl radical (DPPH) method for determining the free radical scavenging activity and 50% inhibition of DPPH radical scavenging effect (IC50) were recorded as 28μg/ml and 35μg/ml for MEMA and standard ascorbic acid. A single dose of alloxan monohydrate (120mg/kg, i.p.) was used to induce diabetes mellitus. The MEMA at the dose of 200 and 400mg/kg and the standard drug glibenclamide (2.5mg/kg) were administered orally to the diabetic rats for 15 days. The FBG levels were estimated on 0, 5, 10 and 15th day. On 15th day, the blood was collected and serum is separated for the estimation of biochemical parameters and the pancreas was isolated for histopathological studies. The results of biochemical parameters and histopathological studies suggest that, MEMA possesses significant antidiabetic property and antioxidant activity compared to ascorbic acid.

Keywords: Morus alba L., Antioxidants, Antidiabetic, Alloxan and Glibenclamide etc.