Abstract

REVIEW ON SPECTROPHOTOMETRIC AND CHROMATOGRAPHIC METHOD DEVELOPMENT AND VALIDATION

Priyanka S. Patil*, Prof. Smita Aher and Prof. Dr. R.S. Bacchav

ABSTRACT

Spectrophotometric and chromatographic is the dominant separation technique to detect separate and quantify the drug. A number of chromatographic parameters were analysed to optimize the method like sample pretreatment, choosing mobile phase, column detector selection. The objective of this article is to review the method development, optimization and validation. Spectrophotometric and chromatographic method development depends on chemical structure of the molecules, synthetic route, solubility, polarity, pH and pKa values and functional group activity etc. validation of thise method as per ICH guidelines gives information regarding various stages and knowing characteristics like accuracy, specificity, linearity, limit of detection, limit of quantification.

Keywords: UV Spectrophotometry, High pressure liquid chromatography (HPLC), Method validation, Method development.