Abstract

CONSTRUCTION, EXPRESSION AND BIOLOGICAL ACTIVITIES OF TAT-SOD1 FUSION PROTEIN

Wu Yi, Wu Li-Xiong*, Guo Yan-Feng and Zhu Yin-Chun

ABSTRACT

Copper/zinc superoxide dismutase (SOD1) has strong antioxidant activity against the reactive oxygen species (ROS). But exogenous SOD1 cannot enter cells because of its low biomembrane permeability. Cell-penetrating peptides (CPPs) can rapidly cross plasma membranes. TAT derived from the HIV-1 virus is a CPP with strong biomembrane permeability. This study aimed to construct a fusion protein TAT-SOD and investigate its antioxidant activity and the membrane penetration efficiency.

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