Abstract

ANTIBACTERIAL ACTIVITY OF WATER FRACTION OF SENDUDUK LEAF EXTRACT (MELASTOMA MALABATHRICUM L.) AGAINST SALMONELLA TYPHI AND STAPHYLOCOCCUS AUREUS

Sri Hainil, Aprilya Sri Rachmayanti*, Helmi Arifin and Estu Peni Kusuma Putri

ABSTRACT

The seated plant is native to tropical Asia, subtropical and Pacific islands. This plant is believed to be a traditional medicine in overcoming indigestion, diarrhea, vaginal discharge, canker sores. The sheltered plant grows wildly empty and sufficiently shielded. This research aims to test the antibacterial activity of water fraction of the leaf extract (Melastoma malabathricum L.) against Salmonella typhi and Staphylococcus aureus bacteria using experimental methods. In this research extraction is carried out using maceration method with ethanol solvent. In testing antibacterial activity using the diffusion method of paper discs with concentrations of 20%, 40%, 60%, 80%, control positive (chloramphenicol 50μg) and negative control (DMSO 5%). Research on antibacterial activity test of water fraction of leaf extract (Melastoma malabathricum L.) against Salmonella typhi and Staphylococcus aureus bacteria produces strong resistance. Based on the results of strong concentration testing is 80% concentration. . In Salmonella typhi bacteria the concentration is 80% with an average of 16.26 mm and positive control 25,53 mm. Staphylococcus aureus bacteria concentration of 80% with an average of 17.63 mm and a positive control of 30,9 mm. Potentially as an antibacterial against Salmonella typhi bacteria and Staphylococcus aureus.

Keywords: Melastoma malabathricum L.; Salmonella typhi; Staphylococcus aureus; antibacterial.