Abstract

EVALUATION OF SOME INFLAMMATORY AND RENAL STATUS IN SYMPTOMATIC HIV-MALARIA CO-INFECTION ON ANTIRETROVIRAL THERAPY IN NAUTH NNEWI, SOUTH EASTERN NIGERIA

Ifeoma Priscilla Ezeugwunne*, Ekuma Sunday Olua, John Ekenedirichukwu Okwara Godwin Aigbedo Aikpitanyi- Iduitua, Chukwujekwu Nwabueze C. Odiegwu, Ubuo Kalu Amah, Blessing K Myke-Mbata, Victor Nwabunwanne Oguaka and Onochie Patrick Manafa

ABSTRACT

Renal and inflammatory status of HIV seropositive individuals are very critical to their health outcome especially when co-infected with Plasmodium falciparum. The aim of this study is to evaluate renal status using alpha-1-microglobulin, beta-2-microglobulin and creatinine as well as the inflammatory levels with the aid of interleukin-6 and interleukin-10 in HIV seropositive individuals on Antiretroviral therapy (ART). A total of 100 participants with the mean age of 37.16±8.84 years were randomly recruited and grouped as: HIV seronegative individuals without malaria parasites (n=25), HIV seropositive individuals on ART with malaria parasites on ART (n=25), HIV seropositive individuals without malaria on ART (n=25) and HIV seronegative individuals with malaria parasites (n=25). 5ml of peripheral whole blood was obtained from each participant for the analyses of CD4 counts, Plasmodium falciparum using standard laboratory methods; level of alpha-1-microglobulin, beta-2-microglobulin, IL-6 and IL-10 using ELISA technique and creatinine level by Jaffe kinetic method. The results showed significant mean differences in serum IL-6. IL-10 and CD4 counts amongst the subjects studied at p< 0.05 respectively. There were significantly higher levels in serum IL-6, IL-10 and significant lower blood CD4 counts in HIV subjects on ART with malaria infection than in control at p<0.05 respectively. Similarly there were significantly higher levels in serum IL-6, IL-10 but significantly lower levels in CD4 counts and β2microglobulin in HIV positive subjects than in control (p<0.05). There were significantly higher levels in serum Creatinine, IL-6, IL-10 but lower CD4 counts in Malaria infected subjects than in control at p<0.05 respectively. Increased IL-6 and IL-10 and lower CD4 in HIV-Malaria co-infection, may lead to inflammatory dysregulation.

Keywords: alpha-1-microglobulin, beta-2-microglobulin, IL-6, IL-10 and CD4.