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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

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Abstract

METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC FOR DETERMINATION OF ASSAY OF MACITENTAN

Aniket P. Kothawade*, Ashwini V. Shelke and Anil G. Jadhav

ABSTRACT

A simple, correct, defined and specific stability indicating RP-HPLC method was developed and validated for the investigation of Macitentan. The chromatographic separation was done in 6 min by Agilent 1100 Series C18 Column (250mm*4.6mm) hypersil ODS column using Acetonitrile: Methanol: water (90:5:5) as a mobile phase and flow rate is set to 1.2ml/min. 211 nm was selected as a detection wavelength. The retention time of macitentan were found to be 2.68 min. 20 μl sample volume was injected into the column at the maintained temperature of 300C. The present method has been validated for Specificity, Stability, Linearity, Precision, and Robustness. The proposed method was effectively functional for the quantitative determination of macitentan in the bulk form.

Keywords: Macitentan, RP-HPLC method, Method development, Validation.

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